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A cell-based evaluation of a non-essential amino acid formulation as a non-bioactive control for activation and stimulation of muscle protein synthesis using ex vivo human serum

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posted on 2022-10-06, 10:53 authored by Bijal Patel, Martina Pauk, Miryam Amigo BenaventMiryam Amigo Benavent, Alice B. Nongonierma, Richard FitzgeraldRichard Fitzgerald, Philip JakemanPhilip Jakeman, BRIAN CARSONBRIAN CARSON
The purpose of this study was to compare the effect of treating skeletal muscle cells with media conditioned by postprandial ex vivo human serum fed with either isonitrogenous Non-Essential Amino Acid (NEAA) or a whey protein hydrolysate (WPH) on stimulating Muscle Protein Synthesis (MPS) in C2C12 skeletal muscle cells. Methods Blood was taken from six young healthy males following overnight fast (fasted) and 60 min postprandial (fed) ingestion of either WPH or NEAA (0.33 g.kg-1 Body Mass). C2C12 myotubes were treated with media conditioned by ex vivo human serum (20%) for 4 h. Activation of MPS signalling (phosphorylation of mTOR, P70S6K and 4E-BP1) were determined in vitro by Western Blot and subsequent MPS were determined in vitro by Western Blot and surface sensing of translation technique (SUnSET) techniques, respectively. Results Media conditioned by NEAA fed serum had no effect on protein signalling or MPS compared to fasted, whereas media conditioned by WPH fed serum significantly increased mTOR (Ser2448), P70S6K and 4E-BP1 phosphorylation (p<0.01, p<0.05) compared to fasted serum. Furthermore, the effect of media conditioned by WPH fed serum on protein signalling and MPS was significantly increased (p<0.01, p<0.05) compared to NEAA fed serum

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Publication

PLoS ONE;

Publisher

Public Library of Science

Note

peer-reviewed

Other Funding information

EI

Language

English

Also affiliated with

  • 4i - Centre for Interventions in Infection, Inflammation & Immunity
  • Health Research Institute (HRI)

Department or School

  • Biological Sciences
  • Physical Education and Sports Science

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