University of Limerick
Browse

Electrochemical detection of Pseudomonas aeruginosa quorum sensing molecules at a liquid | liquid interface

Download (2.07 MB)
journal contribution
posted on 2022-12-02, 15:05 authored by Edward D. Burgoyne, Talia Jane Stockmann, Andrés Molina-Osorio, Rachel Shanahan, Gerard P. McGlacken, Micheál D. Scanlon
Opportunistic pathogenic bacteria, such as Pseudomonas aeruginosa, pose a serious risk to patients suffering from a compromised immune system and those patients with cystic fibrosis. Confirming their presence often requires culturing the bacteria which can take days. Herein is proposed a rapid electrochemical detection method based on P. aeruginosa generated small molecules employed for chemical communication – referred to as quorum sensing (QS) molecules – within the biofilm formed by the bacteria; specifically, 4‐hydroxy‐2‐heptylquinoline (HHQ) and 2‐heptyl‐3,4‐dihydroxyquinoline (PQS, Pseudomonas quinolone signal). This method does not depend on the redox activity of the QS molecules. Instead, as a proof-of-concept, electrochemical monitoring was achieved through aqueous alkali metal ion and proton interfacial complexation with organic solubilized HHQ and PQS at an interface between two immiscible electrolytic solutions (ITIES), specifically, between water and 1,2-dichloroethane. The proton:HHQ and proton:PQS binding stoichiometry’s were discovered to be 1:3 and 1:2, respectively, which is likely due to the relatively high concentrations of QS molecules employed. Owing to the biphasic nature of the methodology, experimental complications due to the poor solubility of the hydrophobic QS molecules in aqueous media were avoided

Funding

EVANS-ALLEN RESEARCH PROGRAM - AR

National Institute of Food and Agriculture

Find out more...

The perceptions of senior management of a semi-state organization on affirmative action

National Research Foundation

Find out more...

Using the Cloud to Streamline the Development of Mobile Phone Apps

Innovate UK

Find out more...

Study on Aerodynamic Characteristics Control of Slender Body Using Active Flow Control Technique

Japan Society for the Promotion of Science

Find out more...

History

Publication

Journal of Physical Chemistry C;123 (40), pp. 24643-24650

Publisher

American Chemical Society

Note

peer-reviewed

Other Funding information

ERC, SFI, IRC, NSERC

Rights

© 2019 ACS This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Physical Chemistry C, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/acs.jpcc.9b08350

Language

English

Also affiliated with

  • Bernal Institute

Department or School

  • Chemical Sciences

Usage metrics

    University of Limerick

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC