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Evaluation of the ethanol tolerance for wild and mutant Synechocystis strains by flow cytometry.

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posted on 2018-03-09, 09:16 authored by Teresa Lopes da Silva, Paula C. Passarinho, Ricardo Galriça, Afonso Zenóglio, Patricia Armshaw, Tony J. Pembroke, CON SHEAHANCON SHEAHAN, Alberto Reis, Francisco Gírio
Flow cytometry was used to evaluate the effect of initial ethanol concentrations on cyanobacterial strains of Synechocystis PCC 6803 [wild-type (WT), and ethanol producing recombinants (UL 004 and UL 030)] in batch cultures. Ethanol recombinants, containing one or two metabolically engineered cassettes, were designed towards the development of an economically competitive process for the direct production of bioethanol from microalgae through an exclusive autotrophic route.It can be concluded that the recombinant Synechocystis UL 030 containing two copies of the genes per genome was the most tolerant to ethanol. Nevertheless, to implement a production process using recombinant strains, the bioethanol produced will be required to be continuously extracted from the culture media via a membrane-based technological process for example to prevent detrimental effects on the biomass. The results presented here are of significance in defining the maximum threshold for bulk ethanol concentration in production media.

Funding

Study on Aerodynamic Characteristics Control of Slender Body Using Active Flow Control Technique

Japan Society for the Promotion of Science

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History

Publication

Biotechnology Reports;17, pp. 137-147

Publisher

Elsevier

Note

peer-reviewed

Other Funding information

ERC

Language

English

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