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Extraction and purification of curcuminoids from crude curcumin by a combination of crystallization and chromatography

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posted on 2022-08-25, 09:49 authored by Claire Heffernan, Benjamin K. Hodnett, Marko Ukrainczyk, Rama Krishna Gamidi, Åke RasmusonÅke Rasmuson
In this work a method is developed for the extraction and purification of the three curcuminoids; Curcumin (CUR), Demethoxycurcumin (DMC) and Bisdemethoxycurcumin (BDMC) from commercially available crude curcumin. In a previous publication the extraction of pure curcumin by repeated crystallization has been described. The focus of this paper is on the following chromatographic treatment of the mother liquor from the crystallization to obtain pure DMC and BDMC, and to increase the yield of pure CUR. In the chromatographic process a mixture of chloroform and methanol is used as the mobile phase and silica gel is used as the stationary phase. Each fraction isolated in the chromatographic process was characterised by High Performance Liquid Chromatography (HPLC) and Mass Spectrometry (LC-MS) techniques, and the pure CUR, DMC and BDMC solid phases were fully characterized by powder Xray diffraction (PXRD), differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA). Stability studies were performed on the purified curcuminoids where the degradation products were observed and analysed by HPLC/LC-MS. Overall, the combined purification method recovered from the crude: 88.5%, 79.7% and 68.8% of CUR, DMC and BDMC respectively, in highly pure form CUR (100%), DMC (98.6%) and BDMC (98.3%).

History

Publication

Organic Process Research and Development;21 (6), pp. 821-826

Publisher

American Chemical Society

Note

peer-reviewed

Other Funding information

SFI

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© Year ACS This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal Title, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.oprd.6b00347

Language

English

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