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Flipping the switch: innovations in inducible probes for protein profiling

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journal contribution
posted on 2022-02-04, 12:06 authored by Sean M. McKenna, Ellen M. Fay, Joanna F. McGouran
Over the past two decades, activity-based probes have enabled a range of discoveries, including the characterization of new enzymes and drug targets. However, their suitability in some labeling experiments can be limited by nonspecific reactivity, poor membrane permeability, or high toxicity. One method for overcoming these issues is through the development of “inducible” activity-based probes. These probes are added to samples in an unreactive state and require in situ transformation to their active form before labeling can occur. In this Review, we discuss a variety of approaches to inducible activity-based probe design, different means of probe activation, and the advancements that have resulted from these applications. Additionally, we highlight recent developments which may provide opportunities for future inducible activity-based probe innovations.

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History

Publication

ACS Chemical Biology;16 (12), pp. 2719-2730

Publisher

American Chemical Society

Note

peer-reviewed

Other Funding information

SFI, IRC

Language

English

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