Silien_2013_high.pdf (1.07 MB)
High resolution imaging with differential infrared absorption micro-spectroscopy
journal contributionposted on 2022-11-23, 11:01 authored by Isabel A. Pita, Nordine Hendaoui, NING LIUNING LIU, Mahendar Kumbham, SYED ANSAR TOFAILSYED ANSAR TOFAIL, André Peremans, Christophe SilienChristophe Silien
Although confocal infrared (IR) absorption micro-spectroscopy is well established for far-field chemical imaging, its scope remains restricted since diffraction limits the spatial resolution to values a little above half the radiation wavelength. Yet, the successful implementations of below-the-diffraction limit far-field fluorescence microscopies using saturated irradiation patterns for example for stimulated-emission depletion and saturated structured-illumination suggest the possibility of using a similar optical patterning strategy for infrared absorption mapping at high resolution. Simulations are used to show that the simple mapping of the difference in transmitted/reflected IR energy between a saturated vortex-shaped beam and a Gaussian reference with a confocal microscope affords the generation of high-resolution vibrational absorption images. On the basis of experimentally relevant parameters, the simulations of the differential absorption scheme reveal a spatial resolution better than a tenth of the wavelength for incident energies about a decade above the saturation threshold. The saturated structured illumination concepts are thus expected to be compatible with the establishment of point-like point-spread functions for measuring the absorbance of samples with a scanning confocal microscope recording the differential transmission/reflection. (C) 2013 Optical Society of America
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PublicationOptics Express, 21 (22), pp. 25632-25642
PublisherOptical Society of America
Other Funding informationCERUNA-FUNDP, INSPIRE, HEA, ERC
RightsThis paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.21.025632. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
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