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Perspectives for the microbial production of methyl propionate integrated with product recovery

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journal contribution
posted on 2018-12-06, 14:21 authored by Joana P.C. Pereira, Luuk A.M. van der Wielen, Adrie J.J. Straathof
A new approach was studied for bio-based production of methyl propionate, a precursor of methyl methacrylate. Recombinant E. coli cells were used to perform a cascade reaction in which 2-butanol is reduced to butanone using alcohol dehydrogenase, and butanone is oxidized to methyl propionate and ethyl acetate using a Baeyer- Villiger monooxygenase (BVMO). Product was removed by in situ stripping. The conversion was in line with a model comprising product formation and stripping kinetics. The maximum conversion rates were 1.14 g-butanone/( L h), 0.11 g-ethyl acetate/(L h), and 0.09 g-methyl propionate/(L h). The enzyme regioselectivity towards methyl propionate was 43% of total ester. Starting from biomass-based production of 2-butanol, full-scale ester production with conventional product purification was calculated to be competitive with petrochemical production if the monooxygenase activity and regioselectivity are enhanced, and the costs of bio-based 2-butanol are minimized.

History

Publication

Bioresouce Technology;256, pp. 187-194

Publisher

Elsevier

Note

peer-reviewed

Other Funding information

Netherlands Organisation for Scientific Research

Language

English

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