University of Limerick
Browse
Silien_2017_spatial.pdf (2.72 MB)

Spatial-domain filter enhanced subtraction microscopy and application to mid-IR imaging

Download (2.72 MB)
journal contribution
posted on 2022-12-08, 16:17 authored by Mahendar Kumbham, RABAH MOURASRABAH MOURAS, Aladin Mani, Susan Daly, Kevin O'Dwyer, Andrea Toma, Paolo Bianchini, Alberto Diaspro, NING LIUNING LIU, Syed A.M. Tofail, Christophe SilienChristophe Silien
We have experimentally investigated the enhancement in spatial resolution by image subtraction in mid-infrared central solid-immersion lens (c-SIL) microscopy. The subtraction exploits a first image measured with the c-SIL point-spread function (PSF) realized with a Gaussian beam and a second image measured with the beam optically patterned by a silicon it-step phase plate, to realize a centrally hollow PSF. The intense sides lobes in both PSFs that are intrinsic to the SIL make the conventional weighted subtraction methods inadequate. A spatial-domain filter with a kernel optimized to match both experimental PSFs in their periphery was thus developed to modify the first image prior to subtraction, and this resulted in greatly improved performance, with polystyrene beads 1.4 0.1 mu m apart optically resolved with a mid-1R wavelength of 3.4 mu m in water. Spatial-domain filtering is applicable to other PSF pairs, and simulations show that it also outperforms conventional subtraction methods for the Gaussian and doughnut beams widely used in visible and near-1R microscopy. (C) 2017 Optical Society of America

Funding

Using the Cloud to Streamline the Development of Mobile Phone Apps

Innovate UK

Find out more...

Study on Aerodynamic Characteristics Control of Slender Body Using Active Flow Control Technique

Japan Society for the Promotion of Science

Find out more...

History

Publication

Optics Express;25 (12), pp. 1345-1352

Publisher

Optical Society of America

Note

peer-reviewed

Other Funding information

ERC, SFI, IRC, INSPIRE

Rights

This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL https://doi.org/10.1364/OE.25.013145 on the OSA website: Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

Language

English

Also affiliated with

  • Bernal Institute

Department or School

  • Physics

Usage metrics

    University of Limerick

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC