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Structural evidence for a reaction intermediate mimic in the active site of a sulfite dehydrogenase†

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journal contribution
posted on 2023-02-24, 09:13 authored by Ahmed Djeghader, Melanie Rossotti, Saleh Abdulkarim, Frederic Biaso, Guillaume Gerbaud, Wolfgang Nitschke, Barbara Schoepp-Cothenet, TEWFIK SOULIMANETEWFIK SOULIMANE, Stephane Grimaldi
By combining X-ray crystallography, electron paramagnetic resonance techniques and density functional theory-based modelling, we provide evidence for a direct coordination of the product analogue, phosphate, to the molybdenum active site of a sulfite dehydrogenase.This interaction is mimicking the still experimentally uncharacterized reaction intermediate proposed to arise during the catalytic cycle of this class of enzymes. This work opens new perspectives for further deciphering the reaction mechanism of this nearly ubiquitous class of oxidoreductases

History

Publication

Chemical Communication;

Publisher

The Royal Society of Chemistry

Note

peer-reviewed

Language

English

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  • Bernal Institute

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  • Chemical Sciences

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