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High resolution imaging with differential infrared absorption micro-spectroscopy
Date
2013
Abstract
Although confocal infrared (IR) absorption micro-spectroscopy is well established for far-field chemical imaging, its scope remains restricted since diffraction limits the spatial resolution to values a little above half the radiation wavelength. Yet, the successful implementations of below-the-diffraction limit far-field fluorescence microscopies using saturated irradiation patterns for example for stimulated-emission depletion and saturated structured-illumination suggest the possibility of using a similar optical patterning strategy for infrared absorption mapping at high resolution. Simulations are used to show that the simple mapping of the difference in transmitted/reflected IR energy between a saturated vortex-shaped beam and a Gaussian reference with a confocal microscope affords the generation of high-resolution vibrational absorption images. On the basis of experimentally relevant parameters, the simulations of the differential absorption scheme reveal a spatial resolution better than a tenth of the wavelength for incident energies about a decade above the saturation threshold. The saturated structured illumination concepts are thus expected to be compatible with the establishment of point-like point-spread functions for measuring the absorbance of samples with a scanning confocal microscope recording the differential transmission/reflection. (C) 2013 Optical Society of America
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Description
peer-reviewed
Publisher
Optical Society of America
Citation
Optics Express, 21 (22), pp. 25632-25642
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Silien_2013_high.pdf
Adobe PDF, 1.07 MB
Keywords
structured-illumination microscopy, raman-scattering microscopy, theoretically unlimited resolution, depletion fluorescence microscopy, optical reconstruction microscopy, vibrational-energy relaxation, ground-state-depletion, synchrotron-radiation, confocal microscopy, diffraction-limit, Chemical sciences
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Funding Information
CERUNA-FUNDP, INSPIRE, Higher Education Authority (HEA), European Research Council (ERC)