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Microindentation of fresh soft biological tissue: A rapid tissue sectioning and mounting protocol
Date
2024
Abstract
Microindentation of fresh biological tissues is necessary for the creation of 3D biomimetic models that accurately represent the native extracellular matrix microenvironment. However, tissue must first be precisely sectioned into slices. Challenges exist in the preparation of fresh tissue slices, as they can tear easily and must be processed rapidly in order to mitigate tissue degradation. In this study, we propose an optimised mounting condition for microindentation and demonstrate that embedding tissue in a mixture of 2.5% agarose and 1.5% gelatin is the most favourable method of tissue slice mounting for microindentation. This protocol allows for rapid processing of fresh biological tissue and is applicable to a variety of tissue types.
Supervisor
Description
Publisher
Public Library of Science
Citation
PLoS ONE, 2024, 19 (2), e0297618
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Funding code
Funding Information
Irish Research Council (IRC) Enterprise Partnership Scheme with Becton Dickinson (EPSPG/2020/467). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.