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Effect of seminal plasma from high- and low-fertility bulls on cauda epididymal sperm function
Date
2017
Abstract
The aim of this study was to characterise the effect of seminal plasma (SP) from bulls of high or low fertility on sperm function. First, the effect of SP on the motility of fresh cauda epididymal spermatozoa (CES) and frozen–thawed ejaculated spermatozoa was assessed (Experiment 1a). Seminal plasma was then collected from bulls of known high and low fertility. Pooled CES were incubated in the SP from each bull, diluted and assessed for motility and viability on Days 1, 2, 3 and 5 after packaging as fresh semen (Experiment 1b). Also assessed were motility, kinematics, viability and mitochondrial membrane potential after thawing (Experiment 1c) as well as hypotonic resistance (Experiment 2) and fertilisation potential using in vitro fertilisation (Experiment 3). Seminal plasma increased the motility of CES (P 0.05). The hypotonic resistance of CES was reduced by SP (P 0.05). In conclusion, SP affects the physiological function of CES but there is no difference between SP from high- or low-fertility bulls.
Supervisor
Description
peer-reviewed
Publisher
CSIRO Publishing
Citation
Reproduction, Fertility and Development;29 (12), pp. 2457-2465
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Files
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Fair_2017_Effect.pdf
Adobe PDF, 266.59 KB
Funding code
Funding Information
Department of Agriculture, Food and the Marine
Sustainable Development Goals
External Link
Type
Article
Rights
https://creativecommons.org/licenses/by-nc-sa/1.0/