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Gene expression analysis of mouse embryonic stem cells following levitation in an ultrasound standing wave trap
Date
2011
Abstract
In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08–0.85 MPa) and times (5–60 min) was performed. Our results showed that levitation of ES cells at the highest employed acoustic pressure for 60 min does not modify gene expression and cells maintain their pluripotency. Embryoid bodies (EBs) also expressed the early and late neural differentiation markers, which were also unaffected by the acoustic field. Our results suggest that the ultrasound trap microenvironment is minimally invasive as the biologic consequences of ES cell replication and EB differentiation proceed without significantly affecting gene expression. The technique holds great promise in safe cell manipulation techniques for a variety of applications including tissue engineering and regenerative medicine.
Supervisor
Description
peer-reviewed
Publisher
Elsevier
Citation
Ultrasound in Medicine & Biology;37 (2), pp. 321-330
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Files
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Wride_2011_Gene.pdf
Adobe PDF, 733.37 KB
Funding code
Funding Information
European Union (EU), Health Research Board (HRB)
Sustainable Development Goals
External Link
Type
Article
Rights
https://creativecommons.org/licenses/by-nc-sa/1.0/