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Date
2024
Abstract
The adaptability and modifiability of nucleic acid structures endow them with distinct advantages in biosensor applications. Nonetheless, improving the accuracy and precision of detection remains a major challenge. Our goal is to create a biosensor system that is highly specific and sensitive by harnessing the customizable and targetable properties of nucleic acids. Initially, Capture-SELEX technique was utilized to isolate DNA aptamers of specifically recognizing small chemical molecules, resulting in versatile probes with fluorescence activation and nuclease activities. Additionally, we engineered a miniaturized six-helix DNA nanopore to enhance the detection sensitivity of trace miRNA. Despite encountering the limited insertion efficiency and conductance overlap, we integrated DNA origami technology to improve the binding efficiency and significantly enhance the detection sensitivity. Ultimately, we believe that the proposed sensing strategy would provide a valuable research paradigm for other nucleic acid-relevant target analysis.
Supervisor
Description
Publisher
University of Limerick
Citation
20th Sensors and Their Applications Conference, 2024, Paper No: 36
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Paper_36.pdf
Adobe PDF, 290.71 KB
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Funding Information
This work was supported by the Natural Science Foundation of China (21575154, 21775160, 81801837, 31800685), the Science Foundation of Jiangsu Province (BE2018665, BK20180250, BK20180258, BK20180261), the CAS/SAFEA International Innovation Teams program, the China Postdoctoral Science Foundation (2017M620228, 2018T110550), University of Bordeaux (department STS), Ligue contre le Cancer (Aquitaine), INSERM (PCSI call) and China Scholarship Council for funding