University of Limerick
Browse
- No file added yet -

Cell specific variation in viability in suspension in in vitro poiseuille flow conditions

Download (1.56 MB)
journal contribution
posted on 2023-01-06, 15:03 authored by Sinéad Á. Connolly, David NewportDavid Newport, Kieran Denis McGourty
The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, while the viability of other cells, which are perhaps less accustomed to suspension in a fluidic environment, is important to retain in flow cytometers and other such devices. Despite this, it is unclear how Poiseuille flow affects cell viability. Following on from previous studies which investigated the viability and inertial positions of circulating breast cancer cells in identical flow conditions, this study investigated the influence that varying flow rate, and the corresponding Reynolds number has on the viability of a range of different circulating cells in laminar pipe flow including primary T cells, primary fibroblasts and neuroblastoma cells. It was found that Reynolds numbers as high as 9.13 had no effect on T-cells while the viabilities of neuroblastoma cells and intestinal fibroblasts were significantly reduced in comparison. This indicates that in vitro flow devices need to be tailored to cell-specific flow regimes

Funding

Using the Cloud to Streamline the Development of Mobile Phone Apps

Innovate UK

Find out more...

History

Publication

Scientific Reports;11, 13997

Publisher

Nature

Note

peer-reviewed

Other Funding information

IRC

Language

English

Also affiliated with

  • Bernal Institute

Department or School

  • School of Engineering

Usage metrics

    University of Limerick

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC