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Electrochemistry of nanozeolite-immobilized cytochrome c in aqueous and nonaqueous solutions

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posted on 2014-10-30, 14:32 authored by Kai Guo, Yuanyuan Hu, Yahong Zhang, Baohong Liu, EDMOND MAGNEREDMOND MAGNER
The electrochemical properties of cytochrome c (cyt c) immobilized on multilayer nanozeolite-modified electrodes have been examined in aqueous and nonaqueous solutions. Layers of Linde type-L zeolites were assembled on indium tin oxide (ITO) glass electrodes followed by the adsorption of cyt c, primarily via electrostatic interactions, onto modified ITO electrodes. The heme protein displayed a quasi-reversible response in aqueous solution with a redox potential of +324 mV (vs NHE), and the surface coverage (Gamma*) increased linearly for the first four layers and then gave a nearly constant value of 200 pmol cm(-2). On immersion of the modified electrodes in 95% (v/v) nonaqueous solutions, the redox potential decreased significantly, a decrease that originated from changes in both the enthalpy and entropy of reduction. On reimmersion of the modified electrode in buffer, the faradic response immediately returned to its original value. These results demonstrate that nanozeolites are potential stable supports for redox proteins and enzymes.

History

Publication

Langmuir;26 (11), pp. 9076-81

Publisher

American Chemical Society

Note

peer-reviewed

Other Funding information

SFI, INSPIRE

Rights

This document is the unedited author's version of a submitted work that was subsequently accepted for publication in Langmuir, copyright © American Chemical Society after peer review. To access the final edited and published work, see http://dx.doi.org/10.1021/la904630c

Language

English

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