Functionalized manganese iron oxide nanoparticles: a dual potential magneto-chemotherapeutic cargo in a 3D breast cancer model
Localized heat generation from manganese iron oxide nanoparticles (MIONPs) conjugated with chemotherapeutics under the exposure of an alternating magnetic field (magneto-chemotherapy) can revolutionize targeted breast cancer therapy. On the other hand, the lack of precise control of local temperature and adequate MIONP distribution in laboratory settings using the conventional two-dimensional (2D) cellular models has limited its further translation in tumor sites. Our current study explored advanced 3D in vitro tumor models as a promising alternative to replicate the complete range of tumor characteristics. Specifically, we have focused on investigating the effectiveness of MIONP-based magneto-chemotherapy (MCT) as an anticancer treatment in a 3D breast cancer model. To achieve this, chitosan-coated MIONPs (CS–MIONPs) are synthesized and functionalized with an anticancer drug (doxorubicin) and a tumor-targeting aptamer (AS1411). CS–MIONPs with a crystallite size of 16.88 nm and a specific absorption rate (SAR) of 181.48 W g−1 are reported. In vitro assessment of MCF-7 breast cancer cell lines in 2D and 3D cell cultures demonstrated anticancer activity. In the 2D and 3D cancer models, the MIONP-mediated MCT reduced cancer cell viability to about 71.48% and 92.2%, respectively. On the other hand, MIONP-mediated MCT under an AC magnetic field diminished spheroids’ viability to 83.76 ± 2%, being the most promising therapeutic modality against breast cancer.
PublicationNanoscale, 2023,15, pp. 15686-15699
PublisherRoyal Society of Chemistry
Other Funding informationAuthor VMK is thankful to the D. Y. Patil Education Society (Deemed to be University), Kolhapur for financial support through the research project (Sanction No. DYPES/DU/R&D/2021/276). Authors, SSP are gratefully thanks for to Chhatrapati Shahu Maharaj Research Training and Human Development Institute (SARTHI), Government of Maharashtra, India for awarding the Junior Research Fellowship (JRF) (Sanction No. CSMNRF-2021/2021-22/896). N. D. Thorat acknowledges funding under the Science Foundation Ireland and Irish Research Council (SFI-IRC) pathway programme (21/ Q9 PATH-S/9634).
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