University of Limerick
Harnedy_2020_Identification.pdf (1.49 MB)

Identification and characterisation of peptides from a boarfish (Capros aper) protein hydrolysate displaying in vitro dipeptidyl peptidase-IV (DPP-IV) inhibitory and insulinotropic activity

Download (1.49 MB)
journal contribution
posted on 2020-04-01, 15:25 authored by Pádraigín A. Harnedy-Rothwell, Chris M. McLaughlin, Martina B. O'Keeffe, Aurélien V. Le Gouic, Philip J. Allsopp, Emeir M. McSorley, Shaun J. Sharkey, Jason Whooley, Brian McGovern, Finbarr P.M. O'Harte, Richard J. Fitzgerald
Twenty-two novel dipeptidyl peptidase-IV (DPP-IV) inhibitory peptides (with IC50 values <200 µM) and fifteen novel insulinotropic peptides were identified in a boarfish protein hydrolysate generated at semi-pilot scale using Alcalase 2.4L and Flavourzyme 500L. This was achieved by bioassay-driven semi preparative reverse phase-high performance liquid chromatography fractionation, liquid chromatography-mass spectrometry and confirmatory studies with synthetic peptides. The most potent DPP-IV inhibitory peptide (IPVDM) had a DPP-IV half maximal inhibitory concentration (IC50) values of 21.72 ± 1.08 µM in a conventional in vitro and 44.26 ± 0.65 µM in an in situ cell-based (Caco 2) DPP-IV inhibition assay. Furthermore, this peptide stimulated potent insulin secretory activity (1.6 fold increase compared to control) from pancreatic BRIN-BD11 cells grown in culture. The tripeptide IPV exhibited potent DPP-IV inhibitory activity (IC50: 5.61 ± 0.20 µM) comparable to that reported for the known DPP-IV inhibitor IPI (IC50: 5.61 µM). Boarfish proteins contain peptide sequences with potential to play a role in glycaemic management in vivo.



Food Research International;131, 108989




peer-reviewed The full text of this article will not be available in ULIR until the embargo expires on the 21/01/2021

Other Funding information

Department of Agriculture, Food and the Marine


This is the author’s version of a work that was accepted for publication in Food Research International. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Food Research International, 2020, 131, 108989,



Usage metrics

    University of Limerick


    No categories selected


    Ref. manager