Abril-Parreño_2021_Identification.pdf (1.49 MB)
Identification and characterization of O-linked glycans in cervical mucus as biomarkers of sperm transport: A novel sheep model
journal contributionposted on 2023-01-12, 16:42 authored by Laura Abril-ParrenoLaura Abril-Parreno, Hayden Wilkinson, Anette Krogenæs, Jack Morgan, Mary E Gallagher, Colm Reid, Xavier Druart, Sean FairSean Fair, Radka Saldov
Cervical mucus plays an important role in female fertility, since it allows the entry of motile and morphological normal sperm while preventing the ascent of pathogens from the vagina. The function of cervical mucus is critically linked to its rheological properties that are in turn dictated by O-glycosylated proteins, called mucins. We aimed to characterize the O-glycan composition in the cervical mucus of six European ewe breeds with known differences in pregnancy rates following cervical/vaginal artificial insemination with frozen–thawed semen, which are due to reported differences in cervical sperm transport. These were Suffolk (low fertility) and Belclare (medium fertility) in Ireland, Ile de France and Romanov (both with medium fertility) in France, and Norwegian White Sheep (NWS) and Fur (both with high fertility) in Norway (n = 28–30 ewes/breed). We identified 124 O-glycans, from which 51 were the major glycans with core 2 and fucosylated glycans as the most common structures. The use of exogenous hormones for synchronization did not affect the O-glycan composition in both high-fertility ewe breeds, but it did in the other four ewe breeds. There was a higher abundance of the sulfated glycan (Galβ1– 3[SO3-GlcNAcβ1–6]GalNAc), fucosylated glycan (GlcNAcβ1–3(Fucα1–2Galβ1–3)GalNAc) and core 4 glycan (GlcNAcβ1–3[GlcNAcβ1–6]GalNAc) in the low-fertility Suffolk breed compared with NWS (high fertility). In addition, core 4 glycans were negatively correlated with mucus viscosity. This novel study has identified O-glycans that are important for cervical sperm transport and could have applications across a range of species including human.
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PublisherOxford University Press
Other Funding informationEuropean Union (EU), Teagasc, Food and Marine, Agence Nationale de la Recherche, Research Council of Norway .
Also affiliated with
- Bernal Institute
Department or School
- Biological Sciences