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Label free detection of specific protein binding using a microwave sensor

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posted on 2022-09-30, 11:23 authored by Marcela Salazar Alvarez, Olga Korostynska, Alex Mason, Ahmed Al Ahamma'a, JAKKI COONEYJAKKI COONEY, EDMOND MAGNEREDMOND MAGNER, SYED ANSAR TOFAILSYED ANSAR TOFAIL
The specific binding of streptavidin to biotinylated protein A was demonstrated using a microwave detection system. In control experiments, the degree of non-specific binding was negligible. The method of detection was used to monitor the adsorption of two other proteins, cytochrome c and glucose oxidase, on to the IDE microwave sensor surface. The response of the sensor was also examined on different substrate materials, with detection of protein binding observed obtained on both smooth, conductive (gold) and on rough, insulating (hydroxyapatite) surfaces.

Funding

Study on Aerodynamic Characteristics Control of Slender Body Using Active Flow Control Technique

Japan Society for the Promotion of Science

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History

Publication

Analyst;139, pp. 5335-5338

Publisher

Royal Society of Chemistry

Note

peer-reviewed

Other Funding information

ERC

Language

English

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