University of Limerick
Carson_2019_Regulation.pdf (1.2 MB)

Regulation of GLUT4 translocation in an in vitro cell model using postprandial human serum ex vivo

Download (1.2 MB)
journal contribution
posted on 2019-09-25, 10:48 authored by Karl E. Cogan, Brian P. Carson, Bijal Patel, Miryam Amigo-Benavent, Philip M. Jakeman, Brendan Egan
Individual amino acids, amino acid mixtures and protein hydrolysates stimulate glucose uptake in many experimental models. To replicate better in vitro the dynamic postprandial response to feeding in vivo, in the present study we investigated the effects of culture media conditioned with fasted and postprandial human serum on GLUT4 translocation in L6‐GLUT4myc myotubes. Serum samples were collected from healthy male participants (n = 8) at baseline (T0), 60 (T60) and 120 min (T120) after the ingestion of 0.33 g (kg body mass)−1 of intact (WPC) or hydrolysed (WPH) whey protein and an isonitrogenous non‐essential amino acid (NEAA) control. L6‐GLUT4myc myotubes were starved of serum and amino acids for 1 h before incubation for 1 h in medium containing 1% postprandial human serum, after which GLUT4 translocation was determined via colorimetric assay. Medium conditioned with fasted human serum at concentrations of 5–20% increased cell surface GLUT4myc abundance. Incubation with serum collected after the ingestion of WPH increased cell surface GLUT4myc at T60 relative to T0 [mean (lower, upper 95% confidence interval)]; [1.13 (1.05, 1.22)], whereas WPC [0.98 (0.90, 1.07)] or NEAA [1.02 (0.94, 1.11)] did not. The differential increases in cell surface GLUT4myc abundance were not explained by differences in serum concentrations of total, essential and branched‐chain amino acids or insulin, glucagon‐like peptide 1 (GLP‐1) and gastric inhibitory polypeptide (GIP). Using a new ex vivo, in vitro approach, cell culture medium conditioned with postprandial serum after the ingestion of a whey protein hydrolysate increased GLUT4 translocation in skeletal muscle cells.



Experimental Physiology;104 (6), pp. 800-807


Wiley and Sons Ltd



Other Funding information

Food for Health Ireland, EI


This is the author accepted peer reviewed version of the following article: Regulation of GLUT4 translocation in an in vitro cell model using postprandial human serum ex vivo, Experimental Physiology, 2019 104 (6), pp 800-807, which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.



Usage metrics

    University of Limerick


    No categories selected


    Ref. manager