University of Limerick
P_Manuscript_Rev3_FINAL_repository.pdf (1.44 MB)

One-time phosphate fertilizer application to grassland columns modifies the soil microbiota and limits its role in ecosystem services

Download (1.44 MB)
journal contribution
posted on 2023-03-02, 14:54 authored by Israel Ikoyi, Andrew FowlerAndrew Fowler, Achim SchmalenbergerAchim Schmalenberger
This study evaluated the effect of one-time phosphate fertilization on the soil microbiota, its cycling of phosphorus (P) and grass growth. Soil columns were established in a greenhouse using a P-limited Irish soil (index 1), planted with Lulium perenne and fertilized with 0 (control), 5 (quarter), 10 (half) and 20 (full) kg P ha(-1) as inorganic phosphate. Only traces of phosphate in soil solution were detected over the 14 week experiment, even after phosphate fertilization. Grass dry matter yield between treatments was not significantly different. Full phosphate fertilization significantly reduced the arbuscular mycorrhization (AM) rate, bacterial- and fungal-feeding nematode population, bacterial phoD gene abundance, but increased alkaline and acid phosphatase activities at the time of harvest. Full and half P treatments significantly shifted the bacterial, fungal and AM community structures compared to the control. Furthermore, the control hail a significantly higher relative abundance of bacterial genera including Bacillus, Bradyrhizobium, Paenibacillus, Nocardioides and Balneimonas, that have been associated with P mobilization in the past, when compared to the full phosphate treatment. These results suggest that a positive effect of a single phosphate application on plant growth in a soil can be cancelled out by its negative effect on the soil microbiota and their ecosystem services. (C) 2018 Elsevier B.V. All rights reserved.



Science of The Total Environment;630, pp. 849-858





Other Funding information



This is the author’s version of a work that was accepted for publication in Science of The Total Environment . Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Science of The Total Environment, 630, pp. 849-858



Department or School

  • Biological Sciences
  • Mathematics & Statistics

Usage metrics

    University of Limerick


    No categories selected