Role of circulating fibrocytes in the diagnosis of acute appendicitis

Background: Improved diagnostic biomarkers are required for acute appendicitis. The circulating fibrocyte percentage (CFP) is increased in inflammatory states, but has not been studied in acute appendicitis. This study aimed to determine CFP in acute appendicitis and compare diagnostic accuracy with standard serological biomarkers. Methods: A prospective cohort study was carried out between June2015 and February2016 at University Hospital Limerick. The CFP was determined by dual-staining peripheral venous samples for CD45 and collagen I using fluorescence-activated cell sorting, and correlated with histopathological diagnoses. The accuracy of CFP in determining histological acute appendicitis was characterized and compared with the white cell count, C-reactive protein concentration, neutrophil count, lymphocyte count and neutrophil:lymphocyte ratio. Results: Of 95 adults recruited, 15 were healthy individuals and 80 had suspected appendicitis at presentation. Forty-six of these 80 patients had an appendicectomy, of whom 34 had histologically confirmed appendicitis. The CFP was statistically higher in patients with pathologically proven acute appendicitis than in healthy controls(median6⋅1 (i.q.r.1⋅6–11⋅6)versus2⋅3(0 ⋅9–3⋅4)percent respectively; P = 0⋅008). The diagnostic accuracy of CFP, as determined using the area under the receiver operating characteristic(ROC)curve, was similar to that of standard biomarkers. In multinomial regression analysis, only raised CFP was retained as an independent prognostic determinant of acute appendicitis (odds ratio 1⋅57, 95 per cent c.i. 1⋅05 to 2⋅33; P = 0⋅027). Conclusion: The CFP is increased in histologically confirmed acute appendicitis and is as accurate as standard serological biomarkers in terms of diagnosis